The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 Encodes a Type III Chaperone and a Cognate Effector

The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 Encodes a Type III Chaperone and a Cognate Effector

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Type III secretion systes are highly conserved among gram-negative plant and sugar skull pet odor candle animal pathogenic bacteria.Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells.Analysis of the whole genome sequence of Pseudomonas syringae pv.

tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P.syringae pv.phaseolicola.

The HopPtoF locus harbors two genes, ShcFPto and HopFPto, that are preceded by a single hrp box promoter.We present evidence here to show that ShcFPto and HopFPto encode a type III chaperone and a cognate effector, respectively.ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell.

Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of plastic molcajete bowls the HopFPto protein to a low level in bacterial cells.